sc 6954 goat anti mouse Search Results


goat anti rrm2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology goat anti rrm2
Goat Anti Rrm2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pmc06155239-310-192-194?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
goat anti rrm2 - by Bioz Stars, 2026-08
96/100 stars
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p53 antibody (pab 240) - bsa free  (Bio-Techne corporation)
94
Bio-Techne corporation p53 antibody (pab 240) - bsa free
P53 Antibody (Pab 240) Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/custom%40nb200-103%4033623049?v=Bio-Techne+corporation
Average 94 stars, based on 1 article reviews
p53 antibody (pab 240) - bsa free - by Bioz Stars, 2026-08
94/100 stars
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goat antimouse 7076 s secondary antibodies  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc goat antimouse 7076 s secondary antibodies
Goat Antimouse 7076 S Secondary Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pmc08256104-38-24-33?v=Cell+Signaling+Technology+Inc
Average 99 stars, based on 1 article reviews
goat antimouse 7076 s secondary antibodies - by Bioz Stars, 2026-08
99/100 stars
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brca1 sc 6954  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology brca1 sc 6954
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
Brca1 Sc 6954, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pmc09413150-55-5-15?v=Santa+Cruz+Biotechnology
Average 96 stars, based on 1 article reviews
brca1 sc 6954 - by Bioz Stars, 2026-08
96/100 stars
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human/mouse/rat sox2 antibody  (Bio-Techne corporation)
96
Bio-Techne corporation human/mouse/rat sox2 antibody
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
Human/Mouse/Rat Sox2 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/custom%40af2018%4032948765?v=Bio-Techne+corporation
Average 96 stars, based on 1 article reviews
human/mouse/rat sox2 antibody - by Bioz Stars, 2026-08
96/100 stars
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53bp1 antibody - bsa free  (Bio-Techne corporation)
98
Bio-Techne corporation 53bp1 antibody - bsa free
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
53bp1 Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/custom%40nb100-304%4010%2E1101%2F508200?v=Bio-Techne+corporation
Average 98 stars, based on 1 article reviews
53bp1 antibody - bsa free - by Bioz Stars, 2026-08
98/100 stars
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dylight 488 affinipure goat anti-rabbit igg (h+l) (e032220)  (Earthox LLC)
90
Earthox LLC dylight 488 affinipure goat anti-rabbit igg (h+l) (e032220)
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
Dylight 488 Affinipure Goat Anti Rabbit Igg (H+L) (E032220), supplied by Earthox LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pmc09413150-55-46-55?v=Earthox+LLC
Average 90 stars, based on 1 article reviews
dylight 488 affinipure goat anti-rabbit igg (h+l) (e032220) - by Bioz Stars, 2026-08
90/100 stars
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rhodamine red x affinipure goat anti mouse  (Jackson Immuno)
95
Jackson Immuno rhodamine red x affinipure goat anti mouse
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
Rhodamine Red X Affinipure Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pmc06716369-981-49-57?v=Jackson+Immuno
Average 95 stars, based on 1 article reviews
rhodamine red x affinipure goat anti mouse - by Bioz Stars, 2026-08
95/100 stars
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α-brdu  (Becton Dickinson)
90
Becton Dickinson α-brdu
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
α Brdu, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pmc06537592-162-14-15?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
α-brdu - by Bioz Stars, 2026-08
90/100 stars
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brca1 sc 6954  (Bethyl)
93
Bethyl brca1 sc 6954
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
Brca1 Sc 6954, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pm37930853-92-87-85?v=Bethyl
Average 93 stars, based on 1 article reviews
brca1 sc 6954 - by Bioz Stars, 2026-08
93/100 stars
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peroxidase affinipure goat  (Jackson Immuno)
96
Jackson Immuno peroxidase affinipure goat
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
Peroxidase Affinipure Goat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pmc08047440-332-64-73?v=Jackson+Immuno
Average 96 stars, based on 1 article reviews
peroxidase affinipure goat - by Bioz Stars, 2026-08
96/100 stars
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alexa fluor 488 affinipure goat anti mouse igg  (Jackson Immuno)
96
Jackson Immuno alexa fluor 488 affinipure goat anti mouse igg
Suppressed <t>BRCA1</t> expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.
Alexa Fluor 488 Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+6954+goat+anti+mouse/pmc13039405-318-68-78?v=Jackson+Immuno
Average 96 stars, based on 1 article reviews
alexa fluor 488 affinipure goat anti mouse igg - by Bioz Stars, 2026-08
96/100 stars
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Image Search Results


Suppressed BRCA1 expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.

Journal: Polymers

Article Title: Comprehensive Analysis of Chromatin Accessibility and Transcriptional Landscape Identified BRCA1 Repression as a Potential Pathological Factor for Keloid

doi: 10.3390/polym14163391

Figure Lengend Snippet: Suppressed BRCA1 expression is a potential pathogenic factor in keloid. ( A ) Protein–protein interaction (PPI) network of DEGs based on STRING database. More lines between two genes indicates more reliable interactions between them, and line color represents evidence type for their interaction. BRCA1 as a hub gene, i.e., highly connected in the network, is highlighted by a red arrow. ( B ) Validation of down-regulation of BRCA1 in keloid DF compared with normal DF, as well as in shBRCA1 and shSCR DF samples by RT-PCR. ( C ) Validation of the suppression of BRCA1 by small hairpin RNA (shRNA) by RT-PCR in normal DF. ( D ) Relative cell viability of normal DF that transfected with shRNA for BRCA1 (shB1) or control (shSCR) quantified by CCK-8 method. ( E ) Wound-healing ability of normal DF that transfected with shB1 or shSCR and quantified by scratch test. The left panel shows the representative DF wound-healing examples and the right panel illustrating the wound closure percent of shB1 and shSCR DF at different time points. Error bars represent the mean ± standard deviation (SD) of three independent experiments. n.s., not significant; * p value < 0.05.

Article Snippet: The following antibodies were used: BRCA1 (sc-6954) and normal mouse immunoglobulin G (IgG) (sc-2025) from Santa Cruz Biotechnology; H3K4me3 (ab8580), H3K4me1 (ab8895), and H3K9me3 (ab8898) from Abcam; H3K27ac (#8173) and normal rabbit IgG (#2729) from Cell Signaling Technology; donkey anti-mouse IgG-AlexaFluor 288 (abs20014) from Abcam; and DyLight 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (E032220) from EarthOx.

Techniques: Expressing, Biomarker Discovery, Reverse Transcription Polymerase Chain Reaction, shRNA, Transfection, Control, CCK-8 Assay, Standard Deviation

BRCA1 associates with molecular events related to keloid formation in normal DF. ( A ) Volcano plot illustrating the log2-based fold change (X-axis) and significance (Y-axis, log10-based p value) of differential expression analysis between shB1 and shSCR DF samples. Blue and red dots are significantly down- and upregulated genes in normal shB1 DF relative to normal shSCR DF, respectively. Dark grey dots represent non-significantly differential expression genes. ( B ) Heatmap of z-score normalized expression values of DEGs in normal shB1 and shSCR DF. ( C ) and ( D ) represent the significantly enriched GO terms and KEGG pathways of DEGs, respectively. CEMT, cardiac epithelial to mesenchymal transition.

Journal: Polymers

Article Title: Comprehensive Analysis of Chromatin Accessibility and Transcriptional Landscape Identified BRCA1 Repression as a Potential Pathological Factor for Keloid

doi: 10.3390/polym14163391

Figure Lengend Snippet: BRCA1 associates with molecular events related to keloid formation in normal DF. ( A ) Volcano plot illustrating the log2-based fold change (X-axis) and significance (Y-axis, log10-based p value) of differential expression analysis between shB1 and shSCR DF samples. Blue and red dots are significantly down- and upregulated genes in normal shB1 DF relative to normal shSCR DF, respectively. Dark grey dots represent non-significantly differential expression genes. ( B ) Heatmap of z-score normalized expression values of DEGs in normal shB1 and shSCR DF. ( C ) and ( D ) represent the significantly enriched GO terms and KEGG pathways of DEGs, respectively. CEMT, cardiac epithelial to mesenchymal transition.

Article Snippet: The following antibodies were used: BRCA1 (sc-6954) and normal mouse immunoglobulin G (IgG) (sc-2025) from Santa Cruz Biotechnology; H3K4me3 (ab8580), H3K4me1 (ab8895), and H3K9me3 (ab8898) from Abcam; H3K27ac (#8173) and normal rabbit IgG (#2729) from Cell Signaling Technology; donkey anti-mouse IgG-AlexaFluor 288 (abs20014) from Abcam; and DyLight 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (E032220) from EarthOx.

Techniques: Quantitative Proteomics, Expressing

BRCA1 might regulate NPTX2 expression by manipulating its enhancer accessibility and influencing enhancer–promoter interactions in normal DF. ( A ) Heatmap of the log2-based fold change of genes that significantly affected differential expression in both shB1 normal DF compared with shSCR normal DF and keloid DF compared with normal DF. LFC, log2-based fold change. ( B ) Validation of down-regulation of NPTX2 in keloid DF relative to normal DF (upper panel) and shB1 normal DF relative to shSCR normal DF (bottom panel). ( C ) Density profile of H3K27ac and H3K4me1 CUT&Tag-seq in keloid DF, ATAC-seq in normal and keloid DF, BRCA1 CUT&Tag-seq in normal and keloid DF at NPTX2 as well as its associated enhancer region. Interaction between NPTX2 enhancer and promoter predicted based on GeneHancer database is shown in bottom. H, normal DF; K, keloid DF. The promoter-associated enhancer that exhibits looser chromatin organization and higher BRCA1 binding signal is highlighted by purple background. ShB1, shBRCA1.

Journal: Polymers

Article Title: Comprehensive Analysis of Chromatin Accessibility and Transcriptional Landscape Identified BRCA1 Repression as a Potential Pathological Factor for Keloid

doi: 10.3390/polym14163391

Figure Lengend Snippet: BRCA1 might regulate NPTX2 expression by manipulating its enhancer accessibility and influencing enhancer–promoter interactions in normal DF. ( A ) Heatmap of the log2-based fold change of genes that significantly affected differential expression in both shB1 normal DF compared with shSCR normal DF and keloid DF compared with normal DF. LFC, log2-based fold change. ( B ) Validation of down-regulation of NPTX2 in keloid DF relative to normal DF (upper panel) and shB1 normal DF relative to shSCR normal DF (bottom panel). ( C ) Density profile of H3K27ac and H3K4me1 CUT&Tag-seq in keloid DF, ATAC-seq in normal and keloid DF, BRCA1 CUT&Tag-seq in normal and keloid DF at NPTX2 as well as its associated enhancer region. Interaction between NPTX2 enhancer and promoter predicted based on GeneHancer database is shown in bottom. H, normal DF; K, keloid DF. The promoter-associated enhancer that exhibits looser chromatin organization and higher BRCA1 binding signal is highlighted by purple background. ShB1, shBRCA1.

Article Snippet: The following antibodies were used: BRCA1 (sc-6954) and normal mouse immunoglobulin G (IgG) (sc-2025) from Santa Cruz Biotechnology; H3K4me3 (ab8580), H3K4me1 (ab8895), and H3K9me3 (ab8898) from Abcam; H3K27ac (#8173) and normal rabbit IgG (#2729) from Cell Signaling Technology; donkey anti-mouse IgG-AlexaFluor 288 (abs20014) from Abcam; and DyLight 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (E032220) from EarthOx.

Techniques: Expressing, Quantitative Proteomics, Biomarker Discovery, Binding Assay